Composite

Part:BBa_K3258030

Designed by: Ayush Pandit   Group: iGEM19_Stanford   (2019-10-21)


MDV + Qbeta + CAM Resistance Gene

Construct with MDV + Qbeta subunit + CAM resistance gene, designed to demonstrate the potential evolution of either or both the Qbeta subunit and the CAM resistance gene. With this construct, Qbeta replicates itself along with the CAM resistance gene, causing CAM resistance which can be tested in vivo, along with its own amplification.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 180
    Illegal XbaI site found at 2786
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 146
    Illegal NotI site found at 2768
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 172
    Illegal BglII site found at 2783
    Illegal BamHI site found at 1538
    Illegal BamHI site found at 1681
    Illegal XhoI site found at 168
    Illegal XhoI site found at 1212
    Illegal XhoI site found at 2777
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 180
    Illegal XbaI site found at 2786
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 180
    Illegal XbaI site found at 2786
    Illegal AgeI site found at 1694
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None